Structure and conformational dynamics of scaffolded DNA origami nanoparticles
نویسندگان
چکیده
منابع مشابه
Structure and conformational dynamics of scaffolded DNA origami nanoparticles
Synthetic DNA is a highly programmable nanoscale material that can be designed to self-assemble into 3D structures that are fully determined by underlying Watson-Crick base pairing. The double crossover (DX) design motif has demonstrated versatility in synthesizing arbitrary DNA nanoparticles on the 5-100 nm scale for diverse applications in biotechnology. Prior computational investigations of ...
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We describe a strategy of scaffolded DNA origami to design and construct 3D molecular cages of tetrahedron geometry with inside volume closed by triangular faces. Each edge of the triangular face is approximately 54 nm in dimension. The estimated total external volume and the internal cavity of the triangular pyramid are about 1.8 x 10(-23) and 1.5 x 10(-23) m(3), respectively. Correct formatio...
متن کاملScaffolded DNA Origami: from Generalized Multicrossovers to Polygonal Networks
My acquaintance with Ned Seeman began in the Caltech library sometime during 1992. At the time I was trying to design a DNA computer and was collecting papers in an attempt to learn all the biochemical tricks ever performed with DNA. Among the papers was Ned and Junghuei Chen's beautiful construction of a DNA cube [1]. I had no idea how to harness such a marvel for computation – the diagrams ex...
متن کاملIn situ structure and dynamics of DNA origami determined through molecular dynamics simulations.
The DNA origami method permits folding of long single-stranded DNA into complex 3D structures with subnanometer precision. Transmission electron microscopy, atomic force microscopy, and recently cryo-EM tomography have been used to characterize the properties of such DNA origami objects, however their microscopic structures and dynamics have remained unknown. Here, we report the results of all-...
متن کاملSupporting Information: In situ structure and dynamics of DNA origami determined through molecular dynamics simulations
Design of the DNA origami structures. Using caDNAno, we designed the following three DNA origami structures: a straight 6by-3 honeycomb-pleated structure (HC), a straight 4-by-4 squarepleated structure (SQ), and a 6-by-3 honeycomb-pleated structure having a 90◦ programmable bend (HC-90◦). Below, we briefly describe our protocols for obtaining the caDNAno designs. Building a DNA origami structur...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2017
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkx378